ELECTROSPRAY MASS-SPECTROMETRY FOR THE CHARACTERIZATION OF THE PURITY OF NATURAL AND MODIFIED OLIGODEOXYNUCLEOTIDES

被引：19

作者：

DEROUSSENT, A ^{[1
]}

LECAER, JP ^{[1
]}

ROSSIER, J ^{[1
]}

GOUYETTE, A ^{[1
]}

机构：

[1] INST ALFRED FESSARD,NEUROPHARMACOL MOLEC LAB,F-91191 GIF SUR YVETTE,FRANCE

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 01期

关键词：

D O I：

10.1002/rcm.1290090102

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrospray ionization mass spectrometry is an accurate and sensitive analytical method to characterize the purity of oligodeoxynucleotides being tested for pharmacological studies. We report the preparation procedure ('desalting') of natural and modified oligodeoxynucleotides (ODNs) and their analysis by negative-ion electrospray mass spectrometry. We evaluate the sensitivity and the accuracy of the method for two antisense ODN sequences. Mass analysis of the 25-mer phosphorothioate can be performed to within 0.001% accuracy (standard error of 0.05 Da) for a sample concentration of 12 pmol/mu L. In addition, the adduct ion and the failure sequence can be identified to characterize the antisense ODN.

引用

页码：1 / 4

页数：4

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