DETECTION OF ROTAVIRUS IN HUMAN STOOLS BY USING MONOCLONAL-ANTIBODY

A monoclonal antibody, 3F7, that reacts with the common rotavirus antigen on the 6th viral gene product was prepared. It was used in a direct monoclonal antibody radioimmunoassay (RIA) as a diagnostic reagent for detection, in 3.5 h, of rotavirus in human pediatric stool specimens. In the 177 samples tested, a concordance of 96% was seen between the monclonal RIA and the well-established and commonly used commercially available Rotazyme test. Six discrepant specimens that were positive by monoclonal RIA but negative by Rotazyme were shown to be positive by EM or confirmatory blocking immunoassay. A 7th discrepant specimen was positive by Rotazyme and negative by monoclonal RIA and by direct and immune electron microscopy. The monoclonal RIA test appears to be highly sensitive and specific, and merits additional evaluation as a rapid, convenient diagnostic assay that can reduce currently encountered problems associated with diagnosing rotavirus infection by immunoassay.