PROCESSING OF YEAST EXOGLUCANASE (BETA-GLUCOSIDASE) IN A KEX2-DEPENDENT MANNER

被引：19

作者：

BASCO, RD ^{[1
]}

GIMENEZGALLEGO, G ^{[1
]}

LARRIBA, G ^{[1
]}

机构：

[1] CSIC,CTR INVEST BIOL,E-28006 MADRID,SPAIN

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Exoglucanase (β-glucosidase); Posttranslational modification; Proteolytic processing; Secretory pathway;

D O I：

10.1016/0014-5793(90)80982-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have detected proteolytic processing of a form of exoglucanase representative of the endoplasmic reticulum (form A). This processing did not take place when form A was obtained from protoplasts lysed in the presence of either EDTA or leupeptin, two wel-characterized inhibitors of KEX2 endoprotease from Saccharomyces cerevisiae. Sequencing of the amino terminus of an A-like form of enzyme secreted by a kex2 mutant indicated the presence of 4 amino acids, with a pair of basic residues (Lys-Arg) at their carboxyl side, preceding the amino terminus of the wild-type external exoglucanase. © 1990.

引用

页码：99 / 102

页数：4

共 20 条

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