STRUCTURAL AND TRANSCRIPTIONAL ANALYSIS OF A HUMAN SUBTELOMERIC REPEAT

被引：21

作者：

CHENG, JF ^{[1
]}

SMITH, CL ^{[1
]}

CANTOR, CR ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 01期

关键词：

D O I：

10.1093/nar/19.1.149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human subtelomeric repeat (designated as the HST repeat) has been isolate and characterized from a yeast artificial chromosome containing one human telomere. This repeat is located immediately adjacent to the telomeric T2AG3 repeats at the extreme termini of the human chromosomes. The DNA sequence of 3.6 kb of the HST repeat has been determined. The HST repeat spans over 3.6 kb in length, and contains one evolutionarily conserved CpG-rich region. The copy number of the HST repeat varies among telomeres. Genomic hybridization experiments suggest that the HST repeat consists of two distinct segments, and the distal portions of the HST repeat are also distributed elsewhere in the genome. In HeLa cells, the HST repeat sequence appears to be transcribed into a 6 kb polyadenylated RNA and a variety of non-polyadenylated RNA species.

引用

页码：149 / 154

页数：6

共 42 条

[1] TELOMERIC REPEAT FROM T-THERMOPHILA CROSS HYBRIDIZES WITH HUMAN TELOMERES [J].

ALLSHIRE, RC ;

GOSDEN, JR ;

CROSS, SH ;

CRANSTON, G ;

ROUT, D ;

SUGAWARA, N ;

SZOSTAK, JW ;

FANTES, PA ;

HASTIE, ND .

NATURE, 1988, 332 (6165) :656-659

[2] HUMAN TELOMERES CONTAIN AT LEAST 3 TYPES OF G-RICH REPEAT DISTRIBUTED NON-RANDOMLY [J].

ALLSHIRE, RC ;

DEMPSTER, M ;

HASTIE, ND .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4611-4627

[3] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[4] IDENTIFICATION OF A TELOMERE-BINDING ACTIVITY FROM YEAST [J].

BERMAN, J ;

TACHIBANA, CY ;

TYE, BK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3713-3717

[5] CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION [J].

BIRD, AP .

NATURE, 1986, 321 (6067) :209-213

[6] THE MOLECULAR-STRUCTURE OF CENTROMERES AND TELOMERES [J].

BLACKBURN, EH ;

SZOSTAK, JW .

ANNUAL REVIEW OF BIOCHEMISTRY, 1984, 53 :163-194

[7]

Borst P, 1981, Cold Spring Harb Symp Quant Biol, V45 Pt 2, P935

[8] MOLECULAR-BASIS FOR TRYPANOSOME ANTIGENIC VARIATION [J].

BORST, P ;

CROSS, GAM .

CELL, 1982, 29 (02) :291-303

[9] STRUCTURE AND POLYMORPHISM OF HUMAN TELOMERE-ASSOCIATED DNA [J].

BROWN, WRA ;

MACKINNON, PJ ;

VILLASANTE, A ;

SPURR, N ;

BUCKLE, VJ ;

DOBSON, MJ .

CELL, 1990, 63 (01) :119-132

[10]

*BROWN WRA, 1989, NATURE, V338, P774

← 1 2 3 4 5 →