IDENTIFICATION OF A PROTEIN FROM SACCHAROMYCES-CEREVISIAE WITH E2F-LIKE DNA-BINDING AND TRANSACTIVATING PROPERTIES

被引：9

作者：

MAI, B ^{[1
]}

LIPP, M ^{[1
]}

机构：

[1] LUDWIG MAXIMILIANS UNIV,INST BIOCHEM,KLOPFERSPITZ 18A,W-8033 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1993年 / 321卷 / 2-3期

关键词：

E2F; MYC; TRANSCRIPTION ACTIVATION; TRANSACTIVATING PROTEIN; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(93)80098-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The promoter of the human proto-oncogene MYC has been the first cellular target shown to be subject to regulation by the E2F transcription factor. E2F also has binding sites in other promoters regulated by cell proliferation and during the cell cycle. We have analyzed Saccharomyces cerevisiae for the presence of an E2F-analogous protein. GAL1-based promoter constructs carrying the E2F binding site of the MYC or the adenovirus E2 promoter showed transcriptional activity in yeast cells. A DNA-binding factor, designated YE2F, binds specifically to the E2F consensus sequence and was partially purified from yeast extracts. YE2F showed identical contact points within the MYC binding site as authentic E2F protein from mammalian cells. The results suggest the existence of an E2F-like protein in the yeast & cerevisae.

引用

页码：153 / 158

页数：6

共 37 条

[1] ADENOVIRUS E1A PROTEINS CAN DISSOCIATE HETEROMERIC COMPLEXES INVOLVING THE E2F TRANSCRIPTION FACTOR - A NOVEL MECHANISM FOR E1A TRANSACTIVATION [J].

BAGCHI, S ;

RAYCHAUDHURI, P ;

NEVINS, JR .

CELL, 1990, 62 (04) :659-669

[2] THE RETINOBLASTOMA PROTEIN COPURIFIES WITH E2F-I, AN E1A-REGULATED INHIBITOR OF THE TRANSCRIPTION FACTOR E2F [J].

BAGCHI, S ;

WEINMANN, R ;

RAYCHAUDHURI, P .

CELL, 1991, 65 (06) :1063-1072

[3] TRANSCRIPTION FACTOR E2F IS REQUIRED FOR EFFICIENT EXPRESSION OF THE HAMSTER DIHYDROFOLATE-REDUCTASE GENE INVITRO AND INVIVO [J].

BLAKE, MC ;

AZIZKHAN, JC .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) :4994-5002

[4] INDEPENDENT BINDING OF THE RETINOBLASTOMA PROTEIN AND P107 TO THE TRANSCRIPTION FACTOR E2F [J].

CAO, L ;

FAHA, B ;

DEMBSKI, M ;

TSAI, LH ;

HARLOW, E ;

DYSON, N .

NATURE, 1992, 355 (6356) :176-179

[5] ADENOVIRUS-E1A, SIMIAN VIRUS-40 TUMOR-ANTIGEN, AND HUMAN PAPILLOMAVIRUS-E7 PROTEIN SHARE THE CAPACITY TO DISRUPT THE INTERACTION BETWEEN TRANSCRIPTION FACTOR-E2F AND THE RETINOBLASTOMA GENE-PRODUCT [J].

CHELLAPPAN, S ;

KRAUS, VB ;

KROGER, B ;

MUNGER, K ;

HOWLEY, PM ;

PHELPS, WC ;

NEVINS, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4549-4553

[6] THE E2F TRANSCRIPTION FACTOR IS A CELLULAR TARGET FOR THE RB PROTEIN [J].

CHELLAPPAN, SP ;

HIEBERT, S ;

MUDRYJ, M ;

HOROWITZ, JM ;

NEVINS, JR .

CELL, 1991, 65 (06) :1053-1061

[7] THE T/E1A-BINDING DOMAIN OF THE RETINOBLASTOMA PRODUCT CAN INTERACT SELECTIVELY WITH A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN [J].

CHITTENDEN, T ;

LIVINGSTON, DM ;

KAELIN, WG .

CELL, 1991, 65 (06) :1073-1082

[8] A CENTRAL ROLE FOR SWI6 IN MODULATING CELL-CYCLE START-SPECIFIC TRANSCRIPTION IN YEAST [J].

DIRICK, L ;

MOLL, T ;

AUER, H ;

NASMYTH, K .

NATURE, 1992, 357 (6378) :508-513

[9]

Dobner T, 1991, DNA Seq, V1, P409, DOI 10.3109/10425179109020799

[10] A CDNA-ENCODING A PRB-BINDING PROTEIN WITH PROPERTIES OF THE TRANSCRIPTION FACTOR E2F [J].

HELIN, K ;

LEES, JA ;

VIDAL, M ;

DYSON, N ;

HARLOW, E ;

FATTAEY, A .

CELL, 1992, 70 (02) :337-350

← 1 2 3 4 →