GABA-LIKE IMMUNOREACTIVITY IN THE GUSTATORY ZONE OF THE NUCLEUS OF THE SOLITARY TRACT IN THE HAMSTER - LIGHT AND ELECTRON-MICROSCOPIC STUDIES

The distribution of GABA-like immunoreactive (GABA-LI) somata was studied in the gustatory zone of the nucleus of the solitary tract (NST) in the hamster in order to identify putative inhibitory circuitry in gustatory processing. Immunoreactive somata were located throughout the gustatory NST, in accordance to the distribution of large and small types of neurons as determined in previous morphometric studies. Consequently, GABA-LI somata were mostly found in the dorsal two-thirds of the gustatory zone. Such somata were mostly ovoid in shape and possessed somal areas that averaged 85.5 +/- 2.8 mum2 (12.7 x 8.4 mum). A narrow range of somal areas (50-125 mum2) suggested a single functional group. At the electron microscopic level, 18% of the neurons encountered were immunoreactive and their nuclei always possessed deeply invaginated boundaries. This morphological feature indicated that GABA-LI neurons are smaller members of the most common class of neurons within the gustatory NST. Because GABA is often implicated as the neurotransmitter of small inhibitory local circuit neurons, these findings indicate a possible inhibitory aspect to the processing of taste information at the level of the first relay in the brainstem.