SEQUENCE-SPECIFIC BINDING OF [N-MECYS3,N-MECYS7]TANDEM TO TPA

被引：9

作者：

LAVESA, M ^{[1
]}

OLSEN, RK ^{[1
]}

FOX, KR ^{[1
]}

机构：

[1] UTAH STATE UNIV,DEPT CHEM & BIOCHEM,LOGAN,UT 84322

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence selective binding of [N-MeCyS3,N-MeCyS7]TANDEM to DNA has been studied by footprinting experiments on DNA fragments containing the self-complementary sequences CGCGATATCGCG,CGCGTATACGCG,CGCGTTAACGCG and CGCGAATTCGCG. DNAase I and micrococcal nuclease reveal drug-induced footprints with the central sequences ATAT, TATA and TTAA, but not AATT, suggesting that the ligand binds to the dinucleotide TpA. The ligand renders certain adenines hyper-reactive to diethyl pyrocarbonate. These are observed with ATAT, TATA and TTAA, but not AATT, and are located both within, and distal to, the TpA-binding sites.

引用

收藏

页码：605 / 607

页数：3

相关论文

共 20 条

[1] PROTON NMR-STUDIES OF [N-MECYS3,N-MECYS7]TANDEM BINDING TO DNA OLIGONUCLEOTIDES - SEQUENCE-SPECIFIC BINDING AT THE TPA SITE [J].

ADDESS, KJ ;

GILBERT, DE ;

OLSEN, RK ;

FEIGON, J .

BIOCHEMISTRY, 1992, 31 (02) :339-350

[2] A METHOD FOR THE SPECIFIC-INHIBITION OF POLY[D(A-T)] SYNTHESIS USING THE A-T SPECIFIC QUINOXALINE ANTIBIOTIC TANDEM [J].

EVANS, DH ;

LEE, JS ;

MORGAN, AR ;

OLSEN, RK .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1982, 60 (02) :131-136

[3] FOOTPRINTING STUDIES ON THE EFFECT OF ECHINOMYCIN ON THE STRUCTURE OF A BENT DNA FRAGMENT [J].

FOX, KR ;

KENTEBE, E .

BIOCHEMICAL JOURNAL, 1990, 269 (01) :217-221

[4] THE USE OF MICROCOCCAL NUCLEASE AS A PROBE FOR DRUG-BINDING SITES ON DNA [J].

FOX, KR ;

WARING, MJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 909 (02) :145-155

[5] NMR-STUDIES OF ECHINOMYCIN BISINTERCALATION COMPLEXES WITH D(AL-C2-G3-T4) AND D(T1-C2-G3-A4) DUPLEXES IN AQUEOUS-SOLUTION - SEQUENCE-DEPENDENT FORMATION OF HOOGSTEEN A1.T4 AND WATSON-CRICK T1.A4 BASE-PAIRS FLANKING THE BISINTERCALATION SITE [J].

GAO, XL ;

PATEL, DJ .

BIOCHEMISTRY, 1988, 27 (05) :1744-1751

[6] THE DNA-SEQUENCE AT ECHINOMYCIN BINDING-SITES DETERMINES THE STRUCTURAL-CHANGES INDUCED BY DRUG-BINDING - NMR-STUDIES OF ECHINOMYCIN BINDING TO [D(ACGTACGT)]2 AND [D(TCGATCGA)]2 [J].

GILBERT, DE ;

FEIGON, J .

BIOCHEMISTRY, 1991, 30 (09) :2483-2494

[7]

LEE JS, 1978, BIOCHEM J, V173, P128

[8] DNA-SEQUENCE RECOGNITION BY UNDER-METHYLATED ANALOGS OF TRIOSTIN-A [J].

LOW, CML ;

FOX, KR ;

OLSEN, RK ;

WARING, MJ .

NUCLEIC ACIDS RESEARCH, 1986, 14 (05) :2015-2033

[9] SEQUENCE PREFERENCES IN THE BINDING TO DNA OF TRIOSTIN-A AND TANDEM AS REPORTED BY DNASE-I FOOTPRINTING [J].

LOW, CML ;

OLSEN, RK ;

WARING, MJ .

FEBS LETTERS, 1984, 176 (02) :414-420

[10] SEQUENCE-SPECIFIC BINDING OF ECHINOMYCIN TO DNA - EVIDENCE FOR CONFORMATIONAL-CHANGES AFFECTING FLANKING SEQUENCES [J].

LOW, CML ;

DREW, HR ;

WARING, MJ .

NUCLEIC ACIDS RESEARCH, 1984, 12 (12) :4865-4879