DIRECT ELECTRON-TRANSFER REACTIONS BETWEEN IMMOBILIZED CYTOCHROME-C AND MODIFIED GOLD ELECTRODES

被引：128

作者：

COOPER, JM ^{[1
]}

GREENOUGH, KR ^{[1
]}

MCNEIL, CJ ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CLIN BIOCHEM,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1993年 / 347卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(93)80094-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper we present data showing quasi-reversible electrochemistry of soluble cytochrome c using the gold electrode modifier N-acetyl cysteine (E1/2 = 25 mV, DELTAE(p) = 60 MV, I(p,a)/v1/2 = constant). Cytochrome c was subsequently immobilized at this modified electrode using a carbodiimide condensation reaction. The electron-transfer rate between the immobilized protein and the gold electrode was estimated as 3.4+/-1.2 s-1 and the formal potential E-degrees' of the immobilized protein electrode was calculated as 2 mV/SCE. We also present data showing an application for the immobilized cytochrome c electrode as a sensor for the specific measurement of superoxide radical production.

引用

页码：267 / 275

页数：9

共 17 条

[1] MECHANISM OF THE REDUCTION AND OXIDATION REACTION OF CYTOCHROME-C AT A MODIFIED GOLD ELECTRODE [J].

ALBERY, WJ ;

EDDOWES, MJ ;

HILL, HAO ;

HILLMAN, AR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1981, 103 (13) :3904-3910

[2] SURFACE SELECTIVITY IN THE DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS - CONTRASTING BEHAVIOR AT EDGE AND BASAL PLANES OF GRAPHITE [J].

ARMSTRONG, FA ;

HILL, HAO ;

OLIVER, BN .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1984, (15) :976-977

[3] ELECTRON-TRANSFER REACTIONS OF METALLOPROTEINS AT PEPTIDE-MODIFIED GOLD ELECTRODES [J].

BARKER, PD ;

DIGLERIA, K ;

HILL, HAO ;

LOWE, VJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 190 (01) :171-175

[4] THE ELECTROCHEMISTRY OF CYTOCHROME-C AT A CONDUCTING POLYMER ELECTRODE [J].

BARTLETT, PN ;

FARINGTON, J .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1989, 261 (2B) :471-475

[5]

Dickerson R.E., 1975, ENZYMES, V11, P397

[6] DIRECT ELECTROCHEMISTRY OF HORSE-HEART CYTOCHROME-C AT AMINO ACID-MODIFIED GOLD ELECTRODES [J].

DIGLERIA, K ;

HILL, HAO ;

LOWE, VJ ;

PAGE, DJ .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1986, 213 (02) :333-338

[7]

EDDOWES MJ, 1977, J CHEM SOC CHEM COMM, P771

[8] METAL-IONS AND OXYGEN RADICAL REACTIONS IN HUMAN INFLAMMATORY JOINT DISEASE [J].

HALLIWELL, B ;

GUTTERIDGE, JMC ;

BLAKE, D .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1985, 311 (1152) :659-671

[9] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].

LAVIRON, E .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28

[10] SPECTRUM OF HORSE-HEART CYTOCHROME-C [J].

MARGOLIASH, E ;

FROHWIRT, N .

BIOCHEMICAL JOURNAL, 1959, 71 :570-578

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