Purification of the rod-shaped nuclear virus (RV-PJ) from kuruma shrimp, Penaeus japonicus

被引：7

作者：

Kimura, T

Nakano, H

Momoyama, K

Yamano, K

Inouye, K

机构：

[1] YAMAGUCHI PREFECTURAL NAIKAI FISHERIES EXPT STN, YAMAGUCHI 754, JAPAN

[2] NATL RES INST AQUACULTURE, INLAND STN, TAMAKI, MIE 51904, JAPAN

来源：

FISH PATHOLOGY | 1995年 / 30卷 / 04期

关键词：

purification; rod-shaped nuclear virus; RVPJ; virus; kuruma shrimp; Penaeus japonicus;

D O I：

10.3147/jsfp.30.287

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Rod-shaped nuclear virus of Penaeus japonicus (RV-PJ) was purified from hemolymph of experimentally infected kuruma shrimp (P. japonicus). The hemolymph collected from infected shrimp was centrifuged at 1,000 x g for 15 min to remove hemocytes and debris. The supernatant was centrifuged at 30,000 x g for 45 min to collect viral particles. The pellet was suspended in 1 ml of PBS (-), and the solution was layered onto a 25-50% (w/w) continuous sucrose gradient. After ultracentrifugation at 125,000 x g for 90 min a single clear band was formed. Rod-shaped virions and nucleocapsids were observed in the band by electron microscopy.

引用

页码：287 / 288

页数：2