CRYSTAL-STRUCTURE OF A CAP-DNA COMPLEX - THE DNA IS BENT BY 90-DEGREES

被引：1037

作者：

SCHULTZ, SC

SHIELDS, GC

STEITZ, TA

机构：

[1] YALE UNIV,DEPT CHEM,NEW HAVEN,CT 06511

[2] YALE UNIV,HOWARD HUGHES MED INST,NEW HAVEN,CT 06511

来源：

SCIENCE | 1991年 / 253卷 / 5023期

关键词：

D O I：

10.1126/science.1653449

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90-degrees. This bend results almost entirely from two 40-degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor-DNA complex provides insights into possible mechanisms of transcription activation.

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页码：1001 / 1007

页数：7

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