CHARACTERIZATION OF A CDNA-ENCODING A HUMAN KIDNEY, CYTOCHROME-P-450 4A FATTY-ACID OMEGA-HYDROXYLASE AND THE COGNATE ENZYME EXPRESSED IN ESCHERICHIA-COLI

被引：81

作者：

PALMER, CNA

RICHARDSON, TH

GRIFFIN, KJ

HSU, MH

MUERHOFF, AS

CLARK, JE

JOHNSON, EF

机构：

[1] SCRIPPS RES INST, DEPT MOLEC & EXPTL MED, DIV BIOCHEM NX4, LA JOLLA, CA 92037 USA

[2] UNIV TEXAS, HLTH SCI CTR, DEPT BIOCHEM, SAN ANTONIO, TX 78284 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1172卷 / 1-2期

关键词：

CYTOCHROME-P-450; CYP4AII; ARACHIDONIC ACID; FATTY ACID; OMEGA-HYDROXYLASE; (HUMAN KIDNEY);

D O I：

10.1016/0167-4781(93)90285-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the omega-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.

引用

页码：161 / 166

页数：6

共 38 条

[1] EXPRESSION AND ENZYMATIC-ACTIVITY OF RECOMBINANT CYTOCHROME-P450 17-ALPHA-HYDROXYLASE IN ESCHERICHIA-COLI [J].