BARRIER-FREE PATHS OF DIRECTED PROTEIN MOTION IN THE ERYTHROCYTE PLASMA-MEMBRANE

被引:18
作者
BOAL, DH
BOEY, SK
机构
[1] Department of Physics, Simon Fraser University, Burnaby, British Columbia
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.1016/S0006-3495(95)79909-X
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A model is presented for the steric interaction between a plasma membrane protein and the membrane cytoskeleton in the human erythrocyte. The cytoskeleton is treated as a network of polymer chains attached to a Rat bilayer, and the membrane protein is a hemisphere of effective radius R(e) with center on the bilayer edge. The simulation is used to investigate the barrier-free path L for linear guided motion of a protein in the bilayer plane. It is shown that the barrier-free paths of small proteins can be used to extract the effective in-plane diameter of cytoskeletal components. For example, the in-plane diameter of an ankyrin attachment site is found to be approximately 12 nm in the simulation, or twice the computational spectrin diameter. The barrier-free paths of large proteins (R(e) > 23 nm) vanish when the proteins are corralled by the cytoskeleton. For intermediate size proteins, L decreases approximately as L proportional to S--1.4 where S is proportional to the sum of the protein and cytoskeleton chain radii.
引用
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页码:372 / 379
页数:8
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