PCR-AMPLIFICATION OF MIXED 16S RIBOSOMAL-RNA GENES FROM AN ANAEROBIC, CYANIDE-DEGRADING CONSORTIUM

被引：10

作者：

BRITSCHGI, TB ^{[1
]}

FALLON, RD ^{[1
]}

机构：

[1] DUPONT CO INC, NEWARK, DE 19714 USA

来源：

FEMS MICROBIOLOGY ECOLOGY | 1994年 / 13卷 / 03期

关键词：

16S RIBOSOMAL-RNA; ANAEROBIC DEGRADATION; CYANID;

D O I：

10.1016/0168-6496(94)90016-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The microorganisms participating in the anaerobic biodegradation of cyanide were characterized using 168 rRNA genes as genetic markers of diversity. Segments of mixed population 16S rRNA genes were amplified using the polymerase chain reaction (PCR) and prokaryote-specific amplification primers. Restriction fragment length polymorphisms (RFLPs) and screening with the 926f universal sequencing primer were used to categorize the cloned PCR products. Six unique prokaryote sequences were obtained, including four similar to methanogens and two similar to Gram-positive eubacteria.

引用

页码：225 / 231

页数：7

共 37 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] GENE ORGANIZATION AND PRIMARY STRUCTURE OF A RIBOSOMAL-RNA OPERON FROM ESCHERICHIA-COLI [J].