COMPARISON OF PERFORMANCES AND ANALYTICAL APPLICATIONS OF 2 IMMOBILIZED OXALATE OXIDASE SENSORS

被引：17

作者：

AMINI, MAS

VALLON, JJ

机构：

[1] HOP EDOUARD HERRIOT,BIOCHIM TOXICOL & ANAL TRACES LAB,LYON,FRANCE

[2] FAC PHARM LYON,CHIM ANALYT LAB 3,LYON,FRANCE

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 299卷 / 01期

关键词：

AMPEROMETRY; ENZYMATIC METHODS; ENZYME ELECTRODE; MEMBRANES; OXALATE OXIDASE;

D O I：

10.1016/0003-2670(94)00322-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The enzyme oxalate oxidase was immobilized on a pre-activated Nylon membrane or on a collagen membrane after activation of the carboxyl groups with the aid of an acyl-azide reaction. Oxalate was quantitated amperometrically via electrooxidation of the hydrogen peroxide generated in the reaction between oxalic acid and oxygen. The specific activities for the collagen and Nylon membranes were 0.13 and 0.74 U/cm(2), respectively. The enzyme activities for the two membranes were determined as a function of pH and ionic strength. The detection limits of oxalate were 1 X 10(-5) M for the collagen sensor and 4 X 10(-7) M for the Nylon sensor.

引用

页码：75 / 79

页数：5

共 17 条

[1]

AMINI MAS, 1989, ANAL LETT, V22, P43

[2] URINARY THIOCYANATE APPEARS TO BE A POWERFUL INHIBITOR OF OXALATE OXIDASE [J].