EXPERIMENTAL STUDIES ON BONE-INDUCING ACTIVITY OF COMPOSITES OF ATELOPEPTIDE TYPE-I COLLAGEN AS A CARRIER FOR ECTOPIC OSTEOINDUCTION BY RHBMP-2

被引：90

作者：

FUJIMURA, K ^{[1
]}

BESSHO, K ^{[1
]}

KUSUMOTO, K ^{[1
]}

OGAWA, Y ^{[1
]}

IIZUKA, T ^{[1
]}

机构：

[1] KANSAI MED UNIV,DEPT PLAST & RECONSTRUCT SURG,MORIGUCHI,OSAKA 570,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1340

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Atelopeptide type I collagen derived from fresh porcine skin was evaluated as a carrier for ectopic osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2) in rats. Four treatment groups (N=5) were examined: a control group in which only atelopeptide type I collagen was implanted, and groups II, III and IV in which atelopeptide type I collagen with 2, 10, 50 mu g of rhBMP-2 was implanted in to the calf muscles of 10-week-old Wistar rats, respectively. Four weeks after the implantation, soft X-ray and light microscopic examinations were performed. In addition, calcium (Ca) content and alkaline phosphatase (ALP) activity were evaluated. New bone formation in the implanted regions (groups II, III and IV) were revealed. Bone formation was induced in the implanted region of even 2 mu g of rhBMP-2 (group II), and its degree was dependent on the dose of rhBMP 2. These results indicate that atelopeptide type I collagen is an effective carrier for ectopic osteoinduction by rhBMP-2 and may act as a carrier for rhBMP in reconstructive surgery for bony defect and augmentation. (C) 1995 Academic Press, Inc.

引用

页码：316 / 322

页数：7