CALCIUM MODULATION OF MITOCHONDRIAL INNER MEMBRANE CHANNEL ACTIVITY

被引：71

作者：

KINNALLY, KW ^{[1
]}

ZOROV, D ^{[1
]}

ANTONENKO, Y ^{[1
]}

PERINI, S ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,DEPT BIOENERGET,MOSCOW 119899,USSR

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(91)90410-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10-7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10-9 to 10-5 M decreased the probability of opening for the MCC and ∼107 pS activities. © 1991.

引用

页码：1183 / 1188

页数：6

共 13 条

[1] ANTONENKO Y N, 1991, Biophysical Journal, V59, p216A
[2] BOWMAN CL, 1983, BIOCHIM BIOPHYS ACTA, V853, P187
[3] ULTRASTRUCTURAL AND BIOCHEMICAL STUDIES OF MITOPLASTS AND OUTER MEMBRANES DERIVED FROM FRENCH-PRESSED MITOCHONDRIA
DECKER, GL
GREENAWALT, JW
[J]. JOURNAL OF ULTRASTRUCTURE RESEARCH, 1977, 59 (01): : 44 - 56
[4] MECHANISMS BY WHICH MITOCHONDRIA TRANSPORT CALCIUM
GUNTER, TE
PFEIFFER, DR
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (05): : C755 - C786
[5] THE MITOCHONDRIAL OUTER-MEMBRANE CHANNEL, VDAC, IS MODULATED BY A SOLUBLE-PROTEIN
HOLDEN, MJ
COLOMBINI, M
[J]. FEBS LETTERS, 1988, 241 (1-2) : 105 - 109
[6] HUNTER DR, 1976, J BIOL CHEM, V251, P5069
[7] MITOCHONDRIAL CHANNEL ACTIVITY STUDIED BY PATCH-CLAMPING MITOPLASTS
KINNALLY, KW
CAMPO, ML
TEDESCHI, H
[J]. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1989, 21 (04) : 497 - 506
[8] MORAN O, 1990, J BIOL CHEM, V265, P908
[9] THE INNER MITOCHONDRIAL-MEMBRANE CONTAINS ION-CONDUCTING CHANNELS SIMILAR TO THOSE FOUND IN BACTERIA
PETRONILLI, V
SZABO, I
ZORATTI, M
[J]. FEBS LETTERS, 1989, 259 (01) : 137 - 143
[10] SANDRI, 1988, CELL CALCIUM, V9, P159

← 1 2 →