EVALUATION OF FLUORESCENT AND COLORED PHOSPHATIDYLCHOLINE ANALOGS AS SUBSTRATES FOR THE ASSAY OF PHOSPHOLIPASE-A2

被引：15

作者：

HENDRICKSON, HS

KOTZ, KJ

HENDRICKSON, EK

机构：

[1] Department of Chemistry, St. Olaf College, Northfield

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90258-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two fluorescent phospholipid analogs, 1-O-{12-(2-naphthyl)-dodec-11-enyl}-2-O-decanoyl-sn-glycero-3-phosphocholine and dansyl-PC [rac 1-O-(N-dansyl-11-amino-1-undecyl)-2-O-decanoyl-glycero-3-phosphocholine], and a red-colored analog, dabsyl-PC [rac 1-O-(N-dabsyl-11-amino-1-undecyl)-2-O-decanoyl-glycero-3-phosphocholine], were evaluated as substrates for the assay of phospholipase A2 (PLA2) (Crotalus adamanteus). The assay reaction was monitored by separation of the fluorescent or colored substrate and product by TLC and quantitation by fluorescence or absorption scanning. All three substrates gave similar (within an order of magnitude) activities with PLA2. Dansyl-PC was best suited to this TLC assay. Dabsyl-PC was less sensitive to detection by absorbance, but had the advantage of being red colored and readily detected by the unaided eye. © 1990.

引用

页码：80 / 83

页数：4

共 10 条

[1]

[Anonymous], 1987, PHOSPHOLIPASES

[2]

HENDRICKSON HS, 1987, J LIPID RES, V28, P864

[3]

JOHNSON JD, 1980, J BIOL CHEM, V255, P3461

[4]

KATES M, 1972, TECHNIQUES LIPIDOLOG, P552