THE EFFECT OF CRYOPRESERVATION ON THE DEVELOPMENT OF S-PHASE AND G2-PHASE MOUSE EMBRYOS

被引：13

作者：

BALAKIER, H

ZENZES, M

WANG, P

MACLUSKY, NJ

CASPER, RF

机构：

[1] Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario

来源：

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1991年 / 8卷 / 02期

关键词：

EMBRYO FREEZING; 3RD-CELL CYCLE; DNA SYNTHESIS; MOUSE EMBRYOS;

D O I：

10.1007/BF01138661

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The survival rate and development of four-cell-stage mouse embryos frozen and thawed in S phase versus G2 phase was compared. Significantly more G2-phase than S-phase embryos survived freezing and thawing. In both groups, disruption of the zona pellucida, fusion of blastomeres, and dispersion of chromosomes were occasionally observed after thawing. Cryopreservation resulted in a longer delay in cleavage from the four- to the eight-cell stage of S (about 5 hr)- and G2-phase embryos (about 3 hr) compared to unfrozen controls. The number of frozen enbryos which developed to the blastocyst stage was reduced compared to controls, and in the case of S-phase embryos, formation of the blastocyst cavity was also delayed. However, the average number of cells in the experimental and control embryos was similar. No increased incidence of chromosome abnormalities was seen. Our results suggest that freezing embryos in G2 is superior to freezing in S phase.

引用

页码：89 / 95

页数：7

共 15 条

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