CONCENTRATING ALKALINE SERINE-PROTEASE, SUBTILISIN, USING A TEMPERATURE-SENSITIVE HYDROGEL

被引：18

作者：

HAN, J

PARK, CH

RUAN, R

机构：

[1] UNIV MINNESOTA, DEPT BIOSYST & AGR ENGN, ST PAUL, MN 55108 USA

[2] KYUNG HEE UNIV, DEPT CHEM ENGN, KYUNGGI DO 449701, SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 08期

关键词：

D O I：

10.1007/BF00129016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The alkaline serine protease, subtilisin, produced by Bacillus licheniformis was concentrated using hydrogel ultrafiltration. Separation efficiency at 15 degrees and 20 degrees C was 84% but decreased above 25 degrees C.

引用

页码：851 / 852

页数：2

共 4 条

[1] A CONTINUOUS CULTURE STUDY OF THE BIOENERGETIC ASPECTS OF GROWTH AND PRODUCTION OF EXOCELLULAR PROTEASE IN BACILLUS-LICHENIFORMIS [J].

FRANKENA, J ;

VANVERSEVELD, HW ;

STOUTHAMER, AH .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 22 (03) :169-176

[2] TEMPERATURE SENSITIVE GELS AS EXTRACTION SOLVENTS [J].

FREITAS, RFS ;

CUSSLER, EL .

CHEMICAL ENGINEERING SCIENCE, 1987, 42 (01) :97-103

[3] CONCENTRATING CELLULASES FROM FERMENTED BROTH USING A TEMPERATURE-SENSITIVE HYDROGEL [J].

PARK, CH ;

OROZCOAVILA, I .

BIOTECHNOLOGY PROGRESS, 1992, 8 (06) :521-526

[4] ESTIMATION OF TEMPERATURE-DEPENDENT PORE-SIZE IN POLY(N-ISOPROPYLACRYLAMIDE) HYDROGEL BEADS [J].

PARK, TG ;

HOFFMAN, AS .

BIOTECHNOLOGY PROGRESS, 1994, 10 (01) :82-86

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