A MODEL OF THE LAC REPRESSOR-OPERATOR COMPLEX BASED ON PHYSICAL AND GENETIC DATA

被引：27

作者：

KISTERSWOIKE, B ^{[1
]}

LEHMING, N ^{[1
]}

SARTORIUS, J ^{[1
]}

VONWILCKENBERGMANN, B ^{[1
]}

MULLERHILL, B ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,WEYERTAL 121,W-5000 COLOGNE 41,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 198卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16030.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Computer graphics were used to build a molecular model of the complex of Lac repressor and lac operator. The model is based (a) on the NMR data of the Kaptein group [Boelens, R., Lamerichs, R. M. J. N., Rullmann, J. A. C., van Boom, J. H. & Kaptein, R. (1988) Protein Sequence Data Anal. 1, 487-498] and (b) on our genetic and biochemical data including specificity changes [Lehming, N., Sartorius, J., Kisters-Woike, B., von Wilcken-Bergmann, B. & Muller-Hill, B. (1990) EMBO J. 9, 615-621]. Effects of amino acid exchanges in the recognition helix could be predicted by the model and were subsequently tested and confirmed by genetic experiments. Comparison of the modelled lac complex with the known crystallographic structures of several helix-turn-helix DNA complexes reveals striking similarities and suggests rules which govern the recognition between particular amino acid side chains and particular base pairs in these systems.

引用

页码：411 / 419

页数：9

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