HB J-ANATOLIA [ALPHA-61(E10)LYS-LESS-THAN-THR] - STRUCTURAL CHARACTERIZATION AND GENE LOCALIZATION OF A NEW ALPHA-CHAIN VARIANT

被引：11

作者：

GIORDANO, PC

FODDE, R

AMONS, R

PLOEM, JE

BERNINI, LF

机构：

[1] LEIDEN STATE UNIV,INST MED BIOCHEM,2312 AV LEIDEN,NETHERLANDS

[2] ST LUCAS HOSP,AMSTERDAM,NETHERLANDS

来源：

HEMOGLOBIN | 1990年 / 14卷 / 02期

关键词：

D O I：

10.3109/03630269009046953

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of a new hemoglobin variant having a single amino acid substitution (Lys Thr) at position 61 of the a chain. In addition to the structural analysis, we also describe the strategy used for the identification of the base substitution and the localization of the defect at the gene level using polymerase chain reaction and hybridization with allele-specific oligonucleotides. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：119 / 128

页数：10

共 14 条

[1]

ABRAHAM E C, 1976, Hemoglobin, V1, P27, DOI 10.3109/03630267609031020

[2] VAPOR-PHASE MODIFICATION OF SULFHYDRYL-GROUPS IN PROTEINS [J].