IS PLASMID BASED DIFFERENTIATION OF COXIELLA-BURNETII IN ACUTE AND CHRONIC ISOLATES STILL VALID

被引:45
作者
THIELE, D
WILLEMS, H
机构
[1] Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Giessen, D-35392
关键词
COXIELLA BURNETII; DIFFERENTIATION; PCR; PLASMID; PULSED FIELD GEL ELECTROPHORESIS; Q FEVER;
D O I
10.1007/BF01719667
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Ten human Coxiella burnetii isolates from french patients with acute hepatitis or chronic endocarditis were characterized according to their polymorphism in DNA restriction patterns and differentiated by plasmid-specific PCR. The aim of this investigation was to clarify if the present classification of so called 'acute' and 'chronic' Coxiella burnetii isolates - based on plasmid profile of a so far limited number of partly ancient isolates - could be confirmed with lately isolated organisms of this agent. The data obtained in this investigation indicate that this classification based on C. burnetii plasmid content is no longer justified.
引用
收藏
页码:427 / 434
页数:8
相关论文
共 16 条
[1]  
Frazier M.E., Mallavia L.P., Samuel J.E., Baca O.G., DNA probes for the identification of Coxiella burnetii strains, NY Acad Sci, 590, pp. 445-458, (1990)
[2]  
Hendrix L.R., Samuel J.E., Mallavia L.P., Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE, J Gen Microbiol, 137, pp. 269-276, (1991)
[3]  
Mallavia L.P., Samuel J.E., Genetic diversity of Coxiella burnetii, Intracellular Parasitism, pp. 117-126, (1989)
[4]  
Samuel J.E., Frazier M.E., Mallavia L.P., Correlation of plasmid type and disease caused by Coxiella burnetii, Infect Immun, 49, pp. 775-779, (1985)
[5]  
Samuel J.E., Frazier M.E., Mallavia L.P., Stability of plasmid sequences in an acute Q-fever strain of Coxiella burnetii, Gen Microbiol, 134, pp. 1795-1805, (1988)
[6]  
Savinelli E.A., Mallavia L.P., Comparison of Coxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate, Ann NY Acad Sci, 590, pp. 523-533, (1990)
[7]  
Thiele D., Wille H., Kopf G., Krauss H., Polymorphism in DNA restriction patterns of Coxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis, Eur J Epidemiol, 9, pp. 419-425, (1993)
[8]  
Vodkin M.H., Willia J.C., Stephenson E.H., Genetic heterogeneity among isolates of Coxiella burnetii, J Gen Microbiol, 132, pp. 455-463, (1986)
[9]  
Wille H., Thiele D., Krauss H., Plasmid based differentiation and detection of Coxiella burnetii in clinical samples, Eur J Epidemiol, 9, pp. 411-418, (1993)
[10]  
Stein A., Raoult D., Lack of pathotype specific gene in human Coxiella burnetii isolates, Microbial Pathogen, 15, pp. 177-185, (1993)