CHARACTERIZATION OF THE POLYHYDROXYALKANOATE SYNTHASE GENE LOCUS OF RHODOBACTER-SPHAEROIDES

被引：25

作者：

HUSTEDE, E ^{[1
]}

STEINBUCHEL, A ^{[1
]}

机构：

[1] UNIV GOTTINGEN,INST MIKROBIOL,GRISEBACHSTR 8,D-37077 GOTTINGEN,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 07期

关键词：

D O I：

10.1007/BF03500683

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Rhodobacter sphaeroides polyhydroxyalkanoate synthase structural gene (phaC(Rs)) was identified as a 1,806-bp open reading frame. It encoded a PHA synthase of 601 amino acids with a relative molecular mass of 66,742, which exhibited 25.9 to 39.0 % amino acid identity with PHA synthases from other bacteria. Enzymatic analysis, nucleotide sequence analysis, hybridization studies and analysis of Tn5-induced PHA-negative mutants provided multiple evidence that phaC(RS) represents a monocistronic operon and that phaC(Rs) is isolated in the genome from other genes known to be relevant for PHA metabolism. It is not clustered with the structural genes for beta-ketothiolase or pyridine nucleotide dependent acetoacetyl-CoA reductase.

引用

页码：709 / 714

页数：6

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