IN-VITRO MODULATION OF CHANGES IN GANGLIOSIDE PATTERNS OF DIFFERENTIATING NEURONS IN THE PRESENCE OF AN ANTI-GM1 ANTIBODY

Retinal cells from 7-day-old chicken embryos were cultured in the presence of a polyclonal anti-GM1 antibody, at low and high density in a ''sandwich cell culture''. Cells that were about 80% neurofilament positive at all times, changed their morphology and emitted processes as controls. By examining immunocytochemical expression of gangliosides, cells cultured in the presence of the antibody maintained GD3 expression longer than controls, albeit the expression of the gangliotetraosylgangliosides (GTOG) was not considerably affected. This leads to an extension of the transient period in which differentiating cells coexpressed both types of gangliosides (GD3 and GTOG). At 3-4 days in vitro the relative synthesis of GD3 was about 30% higher and that of GD1a about 40% lower than in controls, indicating a delay in the shift of the synthesis pattern. Nevertheless, the pattern of ganglioside composition resembled at 4 days in vitro.