MIDKINE IS PRESENT IN THE EARLY-STAGE OF CEREBRAL INFARCT

被引：95

作者：

YOSHIDA, Y

GOTO, M

TSUTSUI, J

OZAWA, M

SATO, E

OSAME, M

MURAMATSU, T

机构：

[1] KAGOSHIMA UNIV, FAC MED, DEPT PATHOL, KAGOSHIMA 890, JAPAN

[2] KAGOSHIMA UNIV, FAC MED, DEPT MED, KAGOSHIMA 890, JAPAN

[3] KAGOSHIMA UNIV, SCH ALLIED MED SCI, KAGOSHIMA 890, JAPAN

[4] NATL LEPROSARIUM HOSHIZUKA KEIAIEN, DIV RES & EXAMINAT, KAGOSHIMA 89321, JAPAN

[5] NAGOYA UNIV, SCH MED, DEPT BIOCHEM, SHOWA KU, NAGOYA, AICHI 466, JAPAN

来源：

DEVELOPMENTAL BRAIN RESEARCH | 1995年 / 85卷 / 01期

关键词：

MIDKINE; CEREBRAL INFARCT; NEUROTROPHIC FACTOR; ASTROCYTE; REPAIR OF BRAIN TISSUE;

D O I：

10.1016/0165-3806(94)00183-Z

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Expression of midkine (MK), a growth factor with neurotrophic activities, was examined immunohistochemically in experimental cerebral infarct of rats. From postoperative day 1 to day 7 after the onset of infarct, anti-MK immunoreactivity was observed in the surrounding ischemic zone of the infarct but not in the necrotic lesion. The immunoreactive material was identified to be MK by Western blotting. On day 14, anti-MK immunoreactivity became negative. Absence of MK in the normal brain was verified both by immunohistochemical staining and Western blotting. The induced expression of MK is an early event: increased expression of glial fibrillary acidic protein (GFAP), a marker of astrocytes, started on day 4 and continued to day 14. These findings suggest that MK is produced around the site of nerve damage and plays a role as a reparative neurotrophic factor during the early phase of cerebral infarct.

引用

页码：25 / 30

页数：6

共 28 条

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