A SULFATED RABBIT ENDOTHELIAL-CELL GLYCOPROTEIN THAT INHIBITS FACTOR-VIIA TISSUE FACTOR IS FUNCTIONALLY AND IMMUNOLOGICALLY IDENTICAL TO RABBIT EXTRINSIC PATHWAY INHIBITOR (EPI)

被引：23

作者：

WARNCRAMER, BJ ^{[1
]}

MAKI, SL ^{[1
]}

RAPAPORT, SI ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT PATHOL,LA JOLLA,CA 92093

来源：

THROMBOSIS RESEARCH | 1991年 / 61卷 / 5-6期

关键词：

EXTRINSIC PATHWAY INHIBITOR; FACTOR-VII; TISSUE FACTOR; FACTOR-X; ENDOTHELIAL CELLS; RABBITS;

D O I：

10.1016/0049-3848(91)90159-T

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Colburn and Buonassisi (In Vitro Cell Dev. Biol. 24, 1133-1136, 1988) have isolated a single chain sulfated glycoprotein inhibitor of factor VIIa/tissue factor-catalyzed activation of factor X from conditioned media of an established rabbit endothelial cell line. We report herein that their endothelial cell-derived inhibitor and extrinsic pathway inhibitor (EPI) isolated from rabbit plasma have identical functional properties with respect to their interactions with factor Xa and with factor VIIa/tissue factor. In addition, the endothelial cell inhibitor and rabbit plasma EPI migrate with the same apparent molecular weights on non-reduced SDS-PAGE and contain similar amounts of N-linked carbohydrate. Like the endothelial cell inhibitor the EPI of rabbit plasma exists as a single chain molecule. Furthermore, the endothelial cell inhibitor is recognized and neutralized by a polyclonal antibody raised against rabbit plasma EPI. We therefore conclude that cultured rabbit endothelial cells produce an inhibitor of factor VIIa/tissue factor activity that is functionally and immunologically identical to rabbit plasma EPI.

引用

页码：515 / 527

页数：13

共 29 条

[1] INHIBITOR OF THE FACTOR-VIIA-TISSUE FACTOR COMPLEX IS REDUCED IN PATIENTS WITH DISSEMINATED INTRAVASCULAR COAGULATION BUT NOT IN PATIENTS WITH SEVERE HEPATOCELLULAR DISEASE [J].