A HIGHLY EFFICIENT ELECTROPORATION SYSTEM FOR TRANSFORMATION OF BACILLUS-LICHENIFORMIS

被引：8

作者：

BRIGIDI, P

DEROSSI, E

RICCARDI, G

MATTEUZZI, D

机构：

[1] UNIV BOLOGNA,DEPT PHARMACEUT SCI,I-40126 BOLOGNA,ITALY

[2] UNIV PAVIA,DEPT GENET & MICROBIOL A BUZZATI TRAVERSO,I-27100 PAVIA,ITALY

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 01期

关键词：

D O I：

10.1007/BF00152745

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 x 10(6), 1.2 x 10(6), and 5.2 x 10(4) transformants per mu-g DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems.

引用

收藏

页码：5 / 8

页数：4

相关论文

共 11 条

[1] TRANSFORMATION OF BACILLUS-CEREUS VEGETATIVE CELLS BY ELECTROPORATION [J].

BELLIVEAU, BH ;

TREVORS, JT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (06) :1649-1652

[2] GENETIC-TRANSFORMATION OF INTACT-CELLS OF BACILLUS-SUBTILIS BY ELECTROPORATION [J].

BRIGIDI, P ;

DEROSSI, E ;

BERTARINI, ML ;

RICCARDI, G ;

MATTEUZZI, D .

FEMS MICROBIOLOGY LETTERS, 1990, 67 (1-2) :135-138

[3] REPLICATION AND EXPRESSION OF PLASMIDS FROM STAPHYLOCOCCUS-AUREUS IN BACILLUS-SUBTILIS [J].

EHRLICH, SD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) :1680-1682

[4] TRANSFORMATION OF BACILLUS LICHENIFORMIS [J].

GWINN, DD ;

THORNE, CB .

JOURNAL OF BACTERIOLOGY, 1964, 87 (03) :519-&

[5] CLONING OF THE GENES FOR PENICILLINASE, PENP AND PENI, OF BACILLUS-LICHENIFORMIS IN SOME VECTOR PLASMIDS AND THEIR EXPRESSION IN ESCHERICHIA-COLI, BACILLUS-SUBTILIS, AND BACILLUS-LICHENIFORMIS [J].

IMANAKA, T ;

TANAKA, T ;

TSUNEKAWA, H ;

AIBA, S .

JOURNAL OF BACTERIOLOGY, 1981, 147 (03) :776-786

[6]

JENSEN KK, 1989, J GEN MICROBIOL, V135, P2283

[7]

Maniatis T., 1982, MOL CLONING

[8] TRANSFORMATION OF VEGETATIVE CELLS OF BACILLUS-ANTHRACIS WITH PLASMID DNA [J].

QUINN, CP ;

DANCER, BN .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :1211-1215

[9] EFFICIENT TRANSFORMATION OF BACILLUS-THURINGIENSIS AND B-CEREUS VIA ELECTROPORATION - TRANSFORMATION OF ACRYSTALLIFEROUS STRAINS WITH A CLONED DELTA-ENDOTOXIN GENE [J].

SCHURTER, W ;

GEISER, M ;

MATHE, D .

MOLECULAR & GENERAL GENETICS, 1989, 218 (01) :177-181

[10]

TAYLOR LD, 1990, FEMS MICROBIOL LETT, V66, P125, DOI [10.1016/0378-1097(90)90269-V, 10.1111/j.1574-6968.1990.tb03983.x]