POLYMERASE CHAIN-REACTION DETECTION OF PLASMODIUM-FALCIPARUM IN MOSQUITOS

被引：38

作者：

TASSANAKAJON, A

BOONSAENG, V

WILAIRAT, P

PANYIM, S

机构：

[1] MAHIDOL UNIV,FAC SCI,DEPT BIOCHEM,BANGKOK 10400,THAILAND

[2] SRINAKHARINWIROT UNIV,FAC MED,DEPT BIOCHEM,BANGKOK,THAILAND

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1993年 / 87卷 / 03期

关键词：

D O I：

10.1016/0035-9203(93)90124-9

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.

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页码：273 / 275

页数：3

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