SEQUENCING OF OLIGONUCLEOTIDES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ELECTROSPRAY MASS-SPECTROMETRY

被引：31

作者：

GLOVER, RP ^{[1
]}

SWEETMAN, GMA ^{[1
]}

FARMER, PB ^{[1
]}

ROBERTS, GCK ^{[1
]}

机构：

[1] UNIV LEICESTER,MRC,TOXICOL UNIT,LEICESTER LE1 9HN,LEICS,ENGLAND

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 10期

关键词：

D O I：

10.1002/rcm.1290091007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.

引用

页码：897 / 901

页数：5

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