STRUCTURE, DISTRIBUTION AND COMPOSITION OF THE EXTRACELLULAR-MATRIX OF HUMAN OOCYTES AND CUMULUS MASSES

被引：67

作者：

DANDEKAR, P ^{[1
]}

AGGELER, J ^{[1
]}

TALBOT, P ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT OBSTET GYNECOL & REPROD MED,SAN FRANCISCO,CA 94143

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 03期

关键词：

HUMAN OOCYTE CUMULUS COMPLEX; HYALURONIC ACID; EXTRACELLULAR MATRIX; FERTILIZATION; HYALURONIDASE;

D O I：

10.1093/oxfordjournals.humrep.a137656

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The structure, distribution and composition of the extracellular matrix present around the human oocyte and in the cumulus was examined following fixation in the presence of ruthenium red. An extracellular matrix comprising granules and filaments is present in the cumulus layer, in the corona radiata, in the outer pores of the zona pellucida and in the perivitelline space surrounding unfertilized oocytes. In replicate samples, the extracellular matrix comprised filaments which were mostly verv long, occasionally cross-connected by shorter filaments, and usually decorated with numerous small granules. Enzymatic digestion with affinity-purified trypsin or Streptomyces hyaluronidase removes the granules and filaments, respectively, at all levels of the oocyte-cumulus complex. These results are interpreted to mean that protein and hyaluronic acid are present in all extracellular compartments of the human oocyte-cumulus complex. The significance of this distribution of hyaluronic acid with respect to the role of sperm hyaluronidase in fertilization is discussed.

引用

页码：391 / 398

页数：8

共 37 条

[1]

Aggeler J., Three-dimensional organization of the extracellular matrix secreted by cultured rat smooth muscle cells, Vitro Cell. Dev. Biol, 24, pp. 633-638, (1988)

[2]

Ball G.D., Beilin M.E., Ax R.L., First N.L., Glycosamino- glycans in bovine cumulus-oocyte complexes: Morphology and chemistry, Mol. Cell. Endocrinol, 28, pp. 113-122, (1982)

[3]

Brown C.R., Distribution of hyaluronidase in the ram spermatozoon, J. Reprod. Fertil, 45, pp. 537-539, (1975)

[4]

Cherr G.N., Lambert H., Meizel S., Katz D.F., In vitro studies of the golden hamster sperm acrosome reaction: Completion on the zona pellucida and induction by homologous soluble zona pellucidae, Dev. Biol, 11, pp. 119-131, (1986)

[5]

Cherr G.N., Yudin A.I., Katz D.F., Organization of the hamster cumulus extracellular matrix: A hyaluronate-glycoprotein gel which modulates sperm access to the oocyte, Dev. Growth Differ, 32, pp. 353-365, (1990)

[6]

Corselli J., Talbot P., In vitro penetration of hamster oocyte- cumulus complexes using physiological numbers of sperm, Dev. Biol, 12, pp. 227-242, (1987)

[7]

Cross N.L., Morales P., Overstreet J.W., Hansen F.W., Induction of acrosome reactions by human zona pellucida, Biol. Reprod, 38, pp. 235-244, (1988)

[8]

Dandekar P., Talbot P., The perivitelline space of mammalian oocytes, Extracellular Matrix of Unfertilized Oocytes and the Formation of a Cortical Granule Envelope Following Fertilization, (1992)

[9]

Dandekar P., Martin M.C., Glass R.G., Polypronuclear embryos after in vitro fertilization, Fertil. Steril, 53, pp. 510-514, (1990)

[10]

Eppig J.J., FSH stimulates hyaluronic acid synthesis by oocyte- cumulus cell complexes from mouse preovulatory follicles, Nature, 28, pp. 483-484, (1979)

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