ORGANIZATION AND TOPOLOGY OF PHOTOSYSTEM-I SUBUNITS

被引:51
作者
ZILBER, AL [1 ]
MALKIN, R [1 ]
机构
[1] UNIV CALIF BERKELEY, DEPT PLANT BIOL, 111 GENET & PLANT BIOL BLDG, BERKELEY, CA 94720 USA
关键词
D O I
10.1104/pp.99.3.901
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Intact spinach (Spinacia oleracea) thylakoid membranes were treated with various proteases and photosystem I (PSI) complexes were isolated from these membranes to define the membrane topology of specific PSI subunits. Trypsin treatment caused cleavage of the PSI-D and E subunits. Thermolysin treatment cleaved the PSI-D, E, H, and K subunits, and also caused limited degradation of the reaction center core PSI-A and B subunits. Pronase treatment produced the most dramatic results as the PSI-A and B subunits were cleaved to 47-, 45-, 26-, and 24-kilodalton products. In addition, pronase degraded the PSI-D, E, H, K, and L subunits. Proteolytic cleavage sites for several of the products were identified by amino acid sequencing. The results indicate that PSI-A, B, D, E, H, K, and L subunits all have stroma-exposed regions, and these findings are summarized in a model describing the subunit organization of PSI.
引用
收藏
页码:901 / 911
页数:11
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