EFFECT OF PRETREATMENT WITH EXCESS SUCROSE OR MANNITOL ON PLANT-REGENERATION FROM RICE CALLUS-CULTURES

被引：9

作者：

HIGUCHI, N

MAEDA, E

机构：

[1] Faculty of Agriculture, Nagoya University, Chikusaku, Nagoya

来源：

JAPANESE JOURNAL OF CROP SCIENCE | 1991年 / 60卷 / 01期

关键词：

CALLUS CULTURE; MANNITOL; ORYZA-SATIVA; PLANT REGENERATION; SCANNING ELECTRON MICROSCOPY; SUCROSE; WATER STRESS;

D O I：

10.1626/jcs.60.122

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The formation of white and compact rice callus cultures was promoted by preculturing mature seed -derived celli on the medium containing a high concentration of sucrose. The obtained white and compact callus had a high regenerating ability on the regeneration medium containing 5 x 10(-5)M kinetin. When mannitol was added to the medium at the same osmotic level as excess sucrose treatment, it offered the intermediate effect between 3% (control) sucrose and 6% (high concentration) sucrose. Scanning electron microscopic examination revealed that the calli which retained the high regenerating ability had a tendency to show a smooth-surfaced appearance.

引用

页码：122 / 129

页数：8

共 34 条

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