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ISOLATION AND AMINO TERMINAL SEQUENCE OF BETA-TRACE, A NOVEL PROTEIN FROM HUMAN CEREBROSPINAL-FLUID

被引：37
作者：
KURUVILLA, AP
HOCHWALD, GM
GHISO, J
CASTANO, EM
PIZZOLATO, M
FRANGIONE, B
机构：
[1] NYU MED CTR,DEPT NEUROL,550 1ST AVE,NEW YORK,NY 10016
[2] NYU MED CTR,DEPT PATHOL,NEW YORK,NY 10016
[3] NYU MED CTR,DEPT PHYSIOL & BIOPHYS,NEW YORK,NY 10016
来源：
BRAIN RESEARCH | 1991年 / 565卷 / 02期
关键词：
HUMAN CEREBROSPINAL FLUID; CEREBROSPINAL FLUID BETA-TRACE PROTEIN; BETA-TRACE ISOLATION; BETA-TRACE; AMINO TERMINAL SEQUENCE;
D O I：
10.1016/0006-8993(91)91666-O
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
beta-Trace, a 23.5 kDa glycoprotein of unknown biological functions, is present in all body fluids tested. It is found in higher concentration in human seminal fluid and cerebrospinal fluid (CSF) than in serum. A one-step procedure for the isolation of beta-trace from pooled CSF is described, by affinity chromatography using a specific antibody made against beta-trace. Amino terminal sequence analysis yields the sequence A P E A Q V S V Q P N F Q Q D K F L G with no homology to known proteins, indicating that beta-trace is a novel CSF protein.
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页码：337 / 340
页数：4
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