PRODUCTION OF SOLAVETIVONE BY IMMOBILIZED CELLS OF HYOSCYAMUS-MUTICUS

被引：10

作者：

RAMAKRISHNA, SV ^{[1
]}

REDDY, GR ^{[1
]}

CURTIS, WR ^{[1
]}

HUMPHREY, AE ^{[1
]}

机构：

[1] CSIR, REG RES LAB, FERMENTAT SECT, TRIVANDRUM 695019, INDIA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 03期

关键词：

D O I：

10.1007/BF00128323

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The secretion of solavetivone, a phytoalexin, by the cells of Hyoscyamus muticus in response to fungal elicitation has been enhanced by gel entrapment in calcium alginate. The immobilized cells produced 53% higher product and exhibited sustained biosynthetic activity in repeated batch cycles when compared with suspended cells. Providing a non-elicited media exchange and a sufficient interval of time between repeated infection (elicitation) gave greater productivity. Apparently the cells need a period to recover from the infection.

引用

页码：301 / 306

页数：6

共 14 条

[1]

ASADA M, 1989, APPL MICROBIOL BIOT, V30, P475, DOI 10.1007/BF00263851

[2] BIOSYNTHESIS AND METABOLISM OF SESQUITERPENOID PHYTOALEXINS AND TRITERPENOIDS IN POTATO CELL-SUSPENSION CULTURES [J].

BRINDLE, PA ;

KUHN, PJ ;

THRELFALL, DR .

PHYTOCHEMISTRY, 1988, 27 (01) :133-150

[3] PRODUCTION OF BIOCHEMICALS WITH IMMOBILIZED PLANT-CELLS - POSSIBILITIES AND PROBLEMS [J].

BRODELIUS, P .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1983, 413 (DEC) :383-393

[4]

BRODELIUS P, 1980, ENZYME ENG, V5, P373

[5] SYNERGISTIC RESPONSE OF PLANT HAIRY-ROOT CULTURES TO PHOSPHATE LIMITATION AND FUNGAL ELICITATION [J].

DUNLOP, DS ;

CURTIS, WR .

BIOTECHNOLOGY PROGRESS, 1991, 7 (05) :434-438

[6]

FARMER EE, 1992, PLANT CELL, V4, P129, DOI 10.1105/tpc.4.2.129

[7] APPROACHES TO UNDERSTANDING AND MANIPULATING THE BIOSYNTHETIC POTENTIAL OF PLANT-ROOTS [J].

FLORES, HE ;

CURTIS, WR .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1992, 665 :188-209

[8] REDIRECTING CELLULAR-METABOLISM BY IMMOBILIZATION OF CULTURED PLANT-CELLS - A MODEL STUDY WITH COFFEA-ARABICA [J].

HALDIMANN, D ;

BRODELIUS, P .

PHYTOCHEMISTRY, 1987, 26 (05) :1431-1434

[9] FUTURE APPROACHES TO THE FORMATION OF SECONDARY NATURAL-PRODUCTS IN PLANT-CELL SUSPENSION-CULTURES [J].

HEINSTEIN, PF .

JOURNAL OF NATURAL PRODUCTS, 1985, 48 (01) :1-9

[10] ENHANCED SHIKONIN PRODUCTION FROM LITHOSPERMUM-ERYTHRORHIZON BY INSITU EXTRACTION AND CALCIUM ALGINATE IMMOBILIZATION [J].

KIM, DJ ;

CHANG, HN .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (05) :460-466

← 1 2 →