INDUCTION OF MESODERM IN XENOPUS-LAEVIS EMBRYOS BY TRANSLATION INITIATION-FACTOR 4E

The microinjection of messenger RNA encoding the eukaryotic translation initiation factor 4E (elF-4E) into early embryos of Xenopus laevis leads to the induction of mesoderm in ectodermal explants. This induction occurs without a stimulation of overall protein synthesis and is blocked by the co-expression of a dominant negative mutant of the proto-oncogene ras or a truncated activin type II receptor. Although other translation factors have been studied in vertebrate and invertebrate embryos, none have been shown to play a direct role in development. The results here suggest a mechanism for relaying and amplifying signals for mesoderm induction.