POLYMERASE CHAIN-REACTION AMPLIFICATION APPLIED TO THE DIRECT DETECTION OF A 4-BP DELETION IN THE PROMOTER REGION OF THE A-GAMMA-GENE

被引：7

作者：

MANCA, L

COCCO, E

MASALA, B

GILMAN, JG

机构：

[1] MONTEFIORE MED CTR,CTR SICKLE CELL,DEPT MED,DIV HEMATOL,111 E 210TH ST,BRONX,NY 10467

[2] UNIV SASSARI,INST GEN PHYSIOL & BIOL CHEM,I-07100 SASSARI,ITALY

来源：

AMERICAN JOURNAL OF HEMATOLOGY | 1990年 / 35卷 / 02期

关键词：

!sup]A[!/sup]γ [!sup]T[!/sup]‐gene; PCR; promoter;

D O I：

10.1002/ajh.2830350215

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We report here the identification of a 4 bp deletion in the AγT globin gene promoter by means of Fnu4Hl digestion of DNA amplified by polymerase chain reaction (PCR). This deletion has been previously associated with haplotype II β‐thalassemia in Sardinia. This simple, non‐radioactive procedure should facilitate the screening of various populations of normal and β‐thalassemic subjects for this specific genetic alteration. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：131 / 133

页数：3

共 10 条

[1]

CAI SP, 1988, BLOOD, V71, P1357

[2] THE DETECTION OF BETA-GLOBIN GENE-MUTATIONS IN BETA-THALASSEMIA USING OLIGONUCLEOTIDE PROBES AND AMPLIFIED DNA [J].