A CALCIUM-SELECTIVE OPTRODE BASED ON THE FLUORESCENCE OF DANSYLATED TROPONIN

被引：8

作者：

ECKERTTILOTTA, SE ^{[1
]}

SCOUTEN, WH ^{[1
]}

HINES, J ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT CHEM,WACO,TX 76798

来源：

APPLIED SPECTROSCOPY | 1991年 / 45卷 / 03期

关键词：

BIOSENSORS; FIBER OPTICS; FLUORESCENCE SPECTROSCOPY; DANSYLAZIRIDINE; TROPONIN; CALCIUM DETERMINATION; OPTRODES;

D O I：

10.1366/0003702914337245

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose. An optrode is constracted by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable. Because fluorescence is enhanced as Ca+2 binds with the immobilized dansylated troponin (TnC(DANZ), this system can be used as a calcium sensor for monitoring plasma Ca+2 levels.

引用

页码：491 / 495

页数：5

共 18 条

[1] BORMAN SA, 1981, ANAL CHEM, V53, pA1616
[2] Ebashi S, 1968, Prog Biophys Mol Biol, V18, P123, DOI 10.1016/0079-6107(68)90023-0
[3] ACTIVE-TRANSPORT OF CALCIUM-ION IN SARCOPLASMIC MEMBRANES
INESI, G
[J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING, 1972, 1 : 191 - 210
[4] INTERACTION OF TROPONIN AND TROPOMYOSIN - SPECTROSCOPIC AND CALORIMETRIC STUDIES
INGRAHAM, RH
SWENSON, CA
[J]. BIOCHEMISTRY, 1985, 24 (19) : 5221 - 5225
[5] JOHNSON JD, 1978, J BIOL CHEM, V253, P6451
[6] JOHNSON JD, 1978, J BIOL CHEM, V253, P5243
[7] LANE H, 1990, AM ASS CLIN CHEM C C
[8] LIO T, 1981, J BIOCH, V90, P163
[9] LIO T, 1980, J BIOCH, V88, P547
[10] PH SENSOR BASED ON IMMOBILIZED FLUORESCEINAMINE
SAARI, LA
SEITZ, WR
[J]. ANALYTICAL CHEMISTRY, 1982, 54 (04) : 821 - 823

← 1 2 →