GENOMIC MAPPING BY ANCHORING RANDOM CLONES - A MATHEMATICAL-ANALYSIS

被引：77

作者：

ARRATIA, R

LANDER, ES

TAVARE, S

WATERMAN, MS

机构：

[1] WHITEHEAD INST BIOMED RES, CAMBRIDGE, MA 02142 USA

[2] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

[3] UNIV SO CALIF, DEPT BIOL SCI, LOS ANGELES, CA 90089 USA

来源：

GENOMICS | 1991年 / 11卷 / 04期

关键词：

D O I：

10.1016/0888-7543(91)90004-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A complete physical map of the DNA of an organism, consisting of overlapping clones spanning the genome, is an extremely useful tool for genomic analysis. Various methods for the construction of such physical maps are available. One approach is to assemble the physical map by "fingerprinting" a large number of random clones and inferring overlap between clones with sufficiently similar fingerprints. E. S. Lander and M. S. Waterman (1988, Genomics 2:231-239) have recently provided a mathematical analysis of such physical mapping schemes, useful for planning such a project. Another approach is to assemble the physical map by "anchoring" a large number of random clones-that is, by taking random short regions called anchors and identifying the clones containing each anchor. Here, we provide a mathematical analysis of such a physical mapping scheme. © 1991.

引用

页码：806 / 827

页数：22

共 10 条

[1] THEORETICAL-ANALYSIS OF A PHYSICAL MAPPING STRATEGY USING RANDOM SINGLE-COPY LANDMARKS [J].