Differential oxidative damage to mitochondrial proteins during aging

被引：47

作者：

Agarwal, S ^{[1
]}

Sohal, RS ^{[1
]}

机构：

[1] SO METHODIST UNIV,DEPT BIOL SCI,DALLAS,TX 75275

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 1995年 / 85卷 / 01期

关键词：

aging; free radicals; mitochondria; oxidative damage; oxidative stress; protein oxidation;

D O I：

10.1016/0047-6374(95)01655-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The level of mitochondrial protein oxidative damage, indicated by the protein carbonyl content, has been previously shown to increase with age. The objective of this study was to determine whether such age-associated damage is random or whether some proteins are relatively more susceptible. Mitochondrial proteins were analyzed by gel filtration. High molecular weight proteins were found to be relatively more oxidatively damaged during aging. Results are interpreted to indicate that protein oxidative damage during aging is not random. The question of the validity of the HPLC method for the measurement of protein carbonyls is discussed in light of a recent report questioning its reliability.

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页码：55 / 63

页数：9

相关论文

共 25 条

[1] AGING AND PROTEIN OXIDATIVE DAMAGE [J].

AGARWAL, S ;

SOHAL, RS .

MECHANISMS OF AGEING AND DEVELOPMENT, 1994, 75 (01) :11-19

[2] DNA OXIDATIVE DAMAGE AND LIFE EXPECTANCY IN HOUSEFLIES [J].

AGARWAL, S ;

SOHAL, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (25) :12332-12335

[3] OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].

AMES, BN ;

SHIGENAGA, MK ;

HAGEN, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7915-7922

[4] ENDOGENOUS OXIDATIVE DNA DAMAGE, AGING, AND CANCER [J].

AMES, BN .

FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 7 (3-6) :121-128

[5]

AMICI A, 1989, J BIOL CHEM, V264, P3341

[6] PROTEIN OXIDATION AND AGING .1. DIFFICULTIES IN MEASURING REACTIVE PROTEIN CARBONYLS IN TISSUES USING 2,4-DINITROPHENYLHYDRAZINE [J].

CAO, GH ;

CUTLER, RG .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 320 (01) :106-114

[7] ARRANGEMENT OF PROTEINS IN THE MITOCHONDRIAL INNER MEMBRANE [J].

CAPALDI, RA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 694 (03) :291-306

[8] OXIDATIVE DAMAGE TO DNA DURING AGING - 8-HYDROXY-2'-DEOXYGUANOSINE IN RAT ORGAN DNA AND URINE [J].

FRAGA, CG ;

SHIGENAGA, MK ;

PARK, JW ;

DEGAN, P ;

AMES, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4533-4537

[9] INACTIVATION OF KEY METABOLIC ENZYMES BY MIXED-FUNCTION OXIDATION REACTIONS - POSSIBLE IMPLICATION IN PROTEIN-TURNOVER AND AGING [J].

FUCCI, L ;

OLIVER, CN ;

COON, MJ ;

STADTMAN, ER .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06) :1521-1525

[10] AGING - A THEORY BASED ON FREE-RADICAL AND RADIATION-CHEMISTRY [J].

HARMAN, D .

JOURNALS OF GERONTOLOGY, 1956, 11 (03) :298-300