GC AND CAPILLARY COLUMN GC/MS DETERMINATION OF SYNTHETIC ANABOLIC-STEROIDS .2. 4-CHLORO-METHANDIENONE (ORAL TURINABOL) AND ITS METABOLITES

The determination of oral turinabol (4-chloro-17 alpha -methyl-17 beta -hydroxy-1,4-androstadien-3-one) in the 'free' fraction of human urine samples by gas chromatography and capillary column gas chromatography/mass spectrometry was studied. After administration to man, three major metabolites are formed whose structures were identified as 6 beta -hydroxy-turinabol left bracket 2 right bracket , 6 beta , 12-dihydroxy-turinabol, and 6 beta , 16-dihydroxy-turinabol, respectively. In much smaller quantities at least another three metabolites are excreted, one of which could be identified as 17-epi-turinabol. No measurable amounts of 1 itself were detected in any of tHE Urine samples investigated. The rate of metabolism and urinary excretion is reasonably fast. The total amount of recovered material, in the form of the three main metabolites, is on the order of 15%. Clean-up procedures and chromatographic conditions are presented in detail.