SEQUENCE AND EXPRESSION OF THE RAT PRODYNORPHIN GENE

被引：386

作者：

CIVELLI, O

DOUGLASS, J

GOLDSTEIN, A

HERBERT, E

机构：

[1] UNIV OREGON, DEPT CHEM, EUGENE, OR 97403 USA

[2] ADDICT RES FDN, PALO ALTO, CA 94304 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1985年 / 82卷 / 12期

关键词：

D O I：

10.1073/pnas.82.12.4291

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The isolation of a .lambda. genomic clone that contains the nucleotide sequence coding for the main exon of the rat prodynorphin (proenkephalin B) gene is reported. This exon codes for the majority of the translated region of prodynorphin mRNA including the opioid peptides .alpha.-neoendorphin, dynorphin A and dynorphin B. The entire 3'' untranslated region is also contained on the .lambda. clone. Nucleotide sequence comparison with the main exon of the human prodynorphin gene reveals both structural and sequence homology. RNA blot analysis reveals that prodynorphin transcripts can be seen in numerous regions of the rat brain and in the adrenal gland, spinal cord, testis and anterior pituitary.

引用

页码：4291 / 4295

页数：5

共 32 条

[1] CIVELLI O, 1982, J BIOL CHEM, V257, P6783
[2] CONE RI, 1983, J NEUROSCI, V3, P2146
[3] COX BM, 1982, REGUL PEPTIDES, P43
[4] ASBESTOS-RELATED INCREASE IN PULMONARY LEVELS OF VASOACTIVE INTESTINAL PEPTIDE (VIP)
DAY, R
LEMAIRE, I
MERCIER, P
BEAUDOIN, H
LEMAIRE, S
[J]. LIFE SCIENCES, 1983, 33 (19) : 1869 - 1876
[5] ISOLATION AND AMINO-ACID SEQUENCE-ANALYSIS OF A 4,000-DALTON DYNORPHIN FROM PORCINE PITUITARY
FISCHLI, W
GOLDSTEIN, A
HUNKAPILLER, MW
HOOD, LE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (17): : 5435 - 5437
[6] DYNORPHIN - A POSSIBLE MODULATORY PEPTIDE ON MORPHINE OR BETA-ENDORPHIN ANALGESIA IN MOUSE
FRIEDMAN, HJ
JEN, MF
CHANG, JK
LEE, NM
LOH, HH
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1981, 69 (03) : 357 - 360
[7] DYNORPHIN-(1-13), AN EXTRAORDINARILY POTENT OPIOID PEPTIDE
GOLDSTEIN, A
TACHIBANA, S
LOWNEY, LI
HUNKAPILLER, M
HOOD, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (12) : 6666 - 6670
[8] PORCINE PITUITARY DYNORPHIN - COMPLETE AMINO-ACID-SEQUENCE OF THE BIOLOGICALLY-ACTIVE HEPTADECAPEPTIDE
GOLDSTEIN, A
FISCHLI, W
LOWNEY, LI
HUNKAPILLER, M
HOOD, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11): : 7219 - 7223
[9] IMMUNOREACTIVE DYNORPHIN IN PITUITARY AND BRAIN
GOLDSTEIN, A
GHAZAROSSIAN, VE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (10): : 6207 - 6210
[10] BEHAVIORAL-EFFECTS AND INVIVO DEGRADATION OF INTRAVENTRICULARLY ADMINISTERED DYNORPHIN-(1-13) AND D-ALA2-DYNORPHIN-(1-11) IN RATS
HERMAN, BH
LESLIE, F
GOLDSTEIN, A
[J]. LIFE SCIENCES, 1980, 27 (10) : 883 - 892

← 1 2 3 4 →