DETERMINATION OF THYROXINE IN URINE BY CATHODIC STRIPPING SQUARE-WAVE VOLTAMMETRY

被引:13
作者
HERNANDEZ, L
HERNANDEZ, P
NIETO, O
机构
关键词
THYROXINE; SQUARE-WAVE VOLTAMMETRY; URINE;
D O I
10.1039/an9941901579
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A square-wave polarographic method for the determination of the amino acid thyroxine in urine, after isolation from the matrix on a C-18 Sep-Pak cartridge and subsequent elution through a column packed with Amberlite IRA-400 anionic resin, was developed. The optimal working conditions for the application of the method were found to be pH 10, provided by a carbonate buffer, and a potential of -0.690 V during 10 min. The equilibration time was applied during 5 s, and potential scans were performed at a square-wave amplitude of 100 mV and a scan rate of 300 mV s(-1), with a square-wave frequency of 100 Hz. The measuring-system response was linear over the thyroxine concentration range from 10 to 60 ng ml(-1) and the detection limit achieved was 2.71 ng ml(-1). The relative error and relative standard deviation obtained were 1.18 and 4.67%, respectively.
引用
收藏
页码:1579 / 1583
页数:5
相关论文
共 58 条
[1]   THE DETERMINATION OF THYROXINE WITH SPECIAL REFERENCE TO TABLETS [J].
ADAMSON, DCM ;
DOMLEO, AP ;
JEFFERIES, JP ;
SHAW, WHC .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 1952, 4 (10) :760-768
[2]  
AIZAWA M, 1987, ELECTROCHEM SENS IMM, P279
[3]  
ALEXANDER NM, 1979, CLIN CHEM, V25, P1757
[4]  
BARD AJ, 1980, ELECTROCHEMICAL METH, P224
[5]   THE CLINICAL DETERMINATION OF PROTEIN-BOUND IODINE [J].
BARKER, SB ;
HUMPHREY, MJ ;
SOLEY, MH .
JOURNAL OF CLINICAL INVESTIGATION, 1951, 30 (01) :55-62
[6]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF IODOAMINO ACIDS FOR TRACER TURNOVER STUDIES OF THYROID-HORMONES INVIVO [J].
BIANCHI, R ;
MOLEA, N ;
CAZZUOLA, F ;
FUSANI, L ;
LOTTI, M ;
BERTELLI, P ;
FERDEGHINI, M ;
MARIANI, G .
JOURNAL OF CHROMATOGRAPHY, 1984, 297 (AUG) :393-398
[7]   DETERMINATION OF LIOTHYRONINE AND THYROXINE IN DRIED THYROID BY GLC [J].
BILOUS, R ;
WINDHEUSER, JJ .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1973, 62 (02) :274-277
[8]   APPLICATION OF THE CERIC SULPHATE-ARSENIOUS ACID REACTION TO THE DETECTION OF THYROXINE AND RELATED SUBSTANCES [J].
BOWDEN, CH ;
MACLAGAN, NF ;
WILKINSON, JH .
BIOCHEMICAL JOURNAL, 1955, 59 (01) :93-97
[9]  
BRENZINA M, 1958, POLAROGRAPHY MED BIO, P216
[10]  
CHEN W, 1984, YAOWU FENXI ZAZHI, V4, P134