SYNTHESIS OF AN ACETYLCHOLINE RECEPTOR-SPECIFIC TOXIN DERIVATIVE REGIOSELECTIVELY LABELED WITH AN UNDECAGOLD CLUSTER

被引：11

作者：

KESSLER, P

KOTZYBAHIBERT, F

LEONETTI, M

BOUET, F

RINGLER, P

BRISSON, A

MENEZ, A

GOELDNER, MP

HIRTH, C

机构：

[1] LAB CHIM BIOORGAN, CNRS, URA 1386, F-67401 ILLKIRCH GRAFFENSTADEN, FRANCE

[2] FAC MED STRASBOURG, INST CHIM BIOL, CNRS, LGME, INSERM, U184, F-67085 STRASBOURG, FRANCE

来源：

BIOCONJUGATE CHEMISTRY | 1994年 / 5卷 / 03期

关键词：

D O I：

10.1021/bc00027a004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The regioselective modification of a snake curaremimetic toxin is described. Toxin-alpha from Naja nigricollis was derivatized with an electron-dense maleimido undecagold cluster 5. The cluster-bound obtained toxin 8 has a very high affinity for the cholinergic binding site (K(i) = 70 pM) of Torpedo marmorata nicotinic receptor. It is harboring a compact heavy atom core of about 8 angstrom which makes it very useful for electron microscopy experiments.

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页码：199 / 204

页数：6

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