MICROSOMAL AND CYTOSOLIC CYTOCHROME-P450 MEDIATED BENZO(A)PYRENE HYDROXYLATION IN PLEUROTUS-PULMONARIUS

被引：37

作者：

MASAPHY, S

LEVANON, D

HENIS, Y

VENKATESWARLU, K

KELLY, SL

机构：

[1] HEBREW UNIV JERUSALEM,FAC AGR,IL-76100 REHOVOT,ISRAEL

[2] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,KREBS INST BIOMOLEC RES,SHEFFIELD S10 2UH,S YORKSHIRE,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 09期

关键词：

D O I：

10.1007/BF00127436

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microsomal and soluble fractions of Pleurotus pulmonarius exhibited a reduced carbon monoxide difference spectrum with P450 maxima at 448nm and 450-452nm respectively. Substrate induced Type I spectra were observed on addition of benzo(a)pyrene to both fractions. Benzo(a)pyrene hydroxylation was measured using the aryl hydrocarbon hydroxylase assay and was observed to be P450 dependent as indicated by carbon monoxide inhibition together with the substrate binding characteristics. The activity of the fractions were observed to give K-m of 200mM and 660mM and V-max of 1.25 nmol/min/nmol P450 and 0.57 nmol/min/nmol P450 for the microsomal and cytosolic fractions respectively.

引用

页码：969 / 974

页数：6

共 18 条

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