MAINTENANCE AND OPERATIONAL STABILITY OF IMMOBILIZED ARTHROBACTER SIMPLEX FOR THE DELTA-1-DEHYDROGENATION OF STEROIDS

被引：14

作者：

KLOOSTERMAN, J ^{[1
]}

LILLY, MD ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL, DEPT CHEM & BIOCHEM ENGN, LONDON WC1E 7JE, ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1985年 / 7卷 / 08期

关键词：

BACTERIOLOGY;

D O I：

10.1016/0141-0229(85)90126-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The stability of the DELTA **1-dehydrogenation system of Arthrobacter simplex immobilized in calcium alginate has been studied. A high stability was related to the ability of the cells to utilize a carbon source such as D-glucose or steroid. Inhibition of de novo protein synthesis reduced the DELTA **1-dehydrogenase stability of the immobilized cells. The operational stability of immobilized cell preparations in the presence of the steroid degradation inhibitor, alpha , alpha -dipyridyl, could not be improved significantly by supplementing steroid substrate suspensions with either D-glucose or yeast extract. (Edited authors abstract).

引用

页码：377 / 382

页数：6

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