PHOSPHORYLATION OF THE NA,K-ATPASE BY CA,PHOSPHOLIPID-DEPENDENT AND CAMP-DEPENDENT PROTEIN-KINASES - MAPPING OF THE REGION PHOSPHORYLATED BY CA,PHOSPHOLIPID-DEPENDENT PROTEIN-KINASE

被引：31

作者：

CHIBALIN, AV ^{[1
]}

LOPINA, OD ^{[1
]}

PETUKHOV, SP ^{[1
]}

VASILETS, LA ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST CHEM PHYS CHERNOGOLOVKA,MOSCOW,RUSSIA

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 1993年 / 25卷 / 01期

关键词：

NA; K-ATPASE; PROTEIN KINASE-C; PROTEIN KINASE-A; PROTEIN PHOSPHORYLATION; PROTEOLYSIS;

D O I：

10.1007/BF00768069

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Ca,phospholipid-dependent (PKC) and cAMP-dependent (PKA) protein kinases phosphorylate the alpha-subunit of the Na,K-ATPase from duck salt gland with the incorporation of 0.3 and 0.5 mol P-32/mol of alpha-subunit, respectively. PKA (in contrast to PKC) phosphorylates the alpha-subunit only in the presence of detergents' Limited tryptic digestion of the Na,K-ATPase phosphorylated by PKC demonstrates that P-32 is incorporated into the N-terminal 41-kDa fragment of the alpha-subunit. Selective chymotrypsin cleavage of phosphorylated enzyme yields a 35-kDa radioactive fragment derived from the central region of the alpha-subunit molecule. These findings suggest that PKC phosphorylates the alpha-subunit of the Na,K-ATPase within the region restricted by C3 and T1 cleavage sites.

引用

页码：61 / 66

页数：6

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