CHARACTERISTICS OF A BRUCELLA SPECIES FROM A BOTTLE-NOSED-DOLPHIN (TURSIOPS-TRUNCATUS)

被引：181

作者：

EWALT, DR

PAYEUR, JB

MARTIN, BM

CUMMINS, DR

MILLER, WG

机构：

[1] US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Veterinary Services Laboratories, Ames

[2] NCCOSC, RTDE DIV511, San Diego

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1994年 / 6卷 / 04期

关键词：

D O I：

10.1177/104063879400600408

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A culture isolated from an aborted fetus of a bottlenose dolphin (Turisops truncatus) was characterized. The isolate was a gram-negative coccobacillus, and the colonial morphology was typical of a smooth Brucella. The isolate was positive for catalase, oxidase, nitrate reduction, and urease. Hydrogen sulfide was not produced. It grew in air at 37 C but required 72 hours for good growth. There was growth on media containing basic fuchsin, thionin, thionin blue, penicillin, and erythritol. The M antigen was dominant, and the isolate was lysed by 4 of 10 brucellaphages tested. The oxidative metabolic profile of the isolate was similar to that for B. abortus but differed in utilization of L-asparagine, L-glutamic acid, and DL-citrulline. Whole-cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein profiles were markedly different from the protein profiles of reference strains of Brucella species. Biochemical and oxidative metabolism profiles indicated that the isolate belongs in the genus Brucella but did not match the profiles of any established species or biovars. This isolate may be an atypical strain of a recognized Brucella species or a new biovar or species of Brucella.

引用

页码：448 / 452

页数：5

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