ISOLATION AND STRUCTURAL CHARACTERIZATION OF A NOVEL PEPTIDE RELATED TO GAMMA-MELANOCYTE STIMULATING HORMONE FROM THE BRAIN OF THE LEECH THEROMYZON TESSULATUM

被引：15

作者：

SALZET, M ^{[1
]}

WATTEZ, C ^{[1
]}

BULET, P ^{[1
]}

MALECHA, J ^{[1
]}

机构：

[1] CNRS,UPR 9022,INST BIOL MOLEC & CELLULAIRE,F-67084 STRASBOURG,FRANCE

来源：

FEBS LETTERS | 1994年 / 348卷 / 01期

关键词：

LEECH; NEUROPEPTIDE; GAMMA-MELANOCYTE STIMULATING HORMONE-LIKE PEPTIDE; INVERTEBRATE; EVOLUTION;

D O I：

10.1016/0014-5793(94)00581-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reports the purification of a novel pro-opiomelanocortin derivative peptide (a gamma-melanocyte stimulating hormone-like (gamma-MSH-like) molecule) from the brain of the leech Theromyzon tessulatum. After reverse-phase HPLC purification, the sequence of the gamma-MSH-like peptide (YVMGHFRWDKFamide) was established by a combination of automated Edman degradation, electrospray mass spectrometry measurement, enzymatic treatment and co-elution experiments in reverse-phase HPLC with synthetic peptides.

引用

页码：102 / 106

页数：5