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NATURE OF ATRIAL NATRIURETIC POLYPEPTIDE IN RAT-BRAIN

被引：126
作者：
SHIONO, S
NAKAO, K
MORII, N
YAMADA, T
ITOH, H
SAKAMOTO, M
SUGAWARA, A
SAITO, Y
KATSUURA, G
IMURA, H
机构：
[1] KYOTO UNIV, SCH MED,DEPT MED,DIV 2,54 SHOGOIN KAWAHARACHO, SAKYO KU, KYOTO 606, JAPAN
[2] KYOTO UNIV, CTR RADIOISOTOPE RES, KYOTO 606, JAPAN
[3] SHIONOGI & CO LTD, SHIONOGI RES LAB, OSAKA 553, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1986年 / 135卷 / 03期
关键词：
D O I：
10.1016/0006-291X(86)90989-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of .alpha.-rat ANP-like immunoreactivity (.alpha.-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with .alpha.-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic .alpha.-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic .alpha.-rANP (4-28) and .alpha.-rANP (5-28), respectively. The peak corresponding to .alpha.-rANP (4-28) was the highest, and only a little .alpha.-rANP-LI was detected at the elution position of .alpha.-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of .alpha.-rANP-LI with a low molecular weight in the rat brain are .alpha.-rAMP (4-28) and .alpha.-rANP (5-28).
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页码：728 / 734
页数：7
相关论文
共 28 条
[1]   PURIFICATION, SEQUENCING AND SYNTHESIS OF NATRIURETIC AND VASOACTIVE RAT ATRIAL PEPTIDE [J].
ATLAS, SA ;
KLEINERT, HD ;
CAMARGO, MJ ;
JANUSZEWICZ, A ;
SEALEY, JE ;
LARAGH, JH ;
SCHILLING, JW ;
LEWICKI, JA ;
JOHNSON, LK ;
MAACK, T .
NATURE, 1984, 309 (5970) :717-719
[2]   A RAPID AND POTENT NATRIURETIC RESPONSE TO INTRAVENOUS-INJECTION OF ATRIAL MYOCARDIAL EXTRACT IN RATS [J].
DEBOLD, AJ ;
BORENSTEIN, HB ;
VERESS, AT ;
SONNENBERG, H .
LIFE SCIENCES, 1981, 28 (01) :89-94
[3]   POST-TRANSLATIONAL PROTEOLYSIS IN POLYPEPTIDE HORMONE BIOSYNTHESIS [J].
DOCHERTY, K ;
STEINER, DF .
ANNUAL REVIEW OF PHYSIOLOGY, 1982, 44 :625-638
[4]   THE AMINO-ACID-SEQUENCE OF AN ATRIAL PEPTIDE WITH POTENT DIURETIC AND NATRIURETIC PROPERTIES [J].
FLYNN, TG ;
DEBOLD, ML ;
DEBOLD, AJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 117 (03) :859-865
[5]   ATRIOPEPTINS - A FAMILY OF POTENT BIOLOGICALLY-ACTIVE PEPTIDES DERIVED FROM MAMMALIAN ATRIA [J].
GELLER, DM ;
CURRIE, MG ;
WAKITANI, K ;
COLE, BR ;
ADAMS, SP ;
FOK, KF ;
SIEGEL, NR ;
EUBANKS, SR ;
GALLUPPI, GR ;
NEEDLEMAN, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (02) :333-338
[6]   MOLECULAR-FORMS OF IMMUNOACTIVE ATRIAL NATRIURETIC PEPTIDE IN THE RAT HYPOTHALAMUS AND ATRIUM [J].
GLEMBOTSKI, CC ;
WILDEY, GM ;
GIBSON, TR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 129 (03) :671-678
[7]  
ITOH H, 1986, JPN HEART J
[8]   EVIDENCE FOR THE EXISTENCE OF ATRIAL NATRIURETIC FACTOR-CONTAINING NEURONS IN THE RAT-BRAIN [J].
JACOBOWITZ, DM ;
SKOFITSCH, G ;
KEISER, HR ;
ESKAY, RL ;
ZAMIR, N .
NEUROENDOCRINOLOGY, 1985, 40 (01) :92-94
[9]   PURIFICATION AND COMPLETE AMINO-ACID-SEQUENCE OF ALPHA-HUMAN ATRIAL NATRIURETIC POLYPEPTIDE (ALPHA-HANP) [J].
KANGAWA, K ;
MATSUO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 118 (01) :131-139
[10]   IDENTIFICATION IN RAT ATRIAL TISSUE OF MULTIPLE FORMS OF NATRIURETIC POLYPEPTIDES OF ABOUT 3000 DALTONS [J].
KANGAWA, K ;
FUKUDA, A ;
KUBOTA, I ;
HAYASHI, Y ;
MATSUO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 121 (02) :585-591
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