ELECTROPHORETIC KARYOTYPE OF CELLULOLYTIC PENICILLIUM-JANTHINELLUM STRAINS

被引：11

作者：

KAYSER, T ^{[1
]}

SCHULZ, G ^{[1
]}

机构：

[1] HUMBOLDT UNIV,INST MIKROBIOL,MAX REIMANN STR 16,O-1532 KLEINMACHNOW,GERMANY

来源：

CURRENT GENETICS | 1991年 / 20卷 / 04期

关键词：

PENICILLIUM-JANTHINELLUM; ELECTROPHORETIC KARYOTYPE; PULSE FIELD GEL ELECTROPHORESIS; GENOME MAPPING; CELLULASE;

D O I：

10.1007/BF00318517

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genome of the cellulolytic fungus Penicillium janthinellum BIOURGE was resolved completely by rotating field electrophoresis. The gel pattern revealed 8-10 different chromosomes. On the basis of data for yeast chromosome size standards from Schizosaccharomyces pombe and Saccharomyces cerevisiae, chromosome sizes in the range from 2.0 to 8 mb were estimated. By Southern hybridization with heterologous probes, the chromosomal locations of rDNA, the elongation factor EF1, actin and ubiquitin have all been mapped. In addition, first results with heterologous and homologous probes for cellulolytic genes from Trichoderma reesei and P. janthinellum are presented. In contrast to the other probes, signals obtained with cellulase genes are clearly distributed over several chromosomes.

引用

页码：289 / 291

页数：3

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